MSE PRO Human Irisin ELISA Kit

Test Quantity:

Product name	Human Irisin ELISA Kit
Reactivity	Human
Alternative Names	FRCP2; Irisin; Fibronectin type III repeat-containing protein 2; FNDC5; Fibronectin Type III Domain Containing Protein 5
Assay Type	Sandwich
Sensitivity	0.11 ng/mL
Standard	20 ng/mL
Detection Range	0.32-20 ng/mL
Sample type	serum, plasma, tissue homogenates and other biological fluids
Assay length	3.5h
Research Area	CD & Adhesion molecule
Test principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Irisin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Irisin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Irisin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Human Irisin in the samples is then determined by comparing the OD of the samples to the standard curve.

Original price was: $336.79.Current price is: $148.00.