Tribioscience Glucose dehydrogenase(NAD(P)-dependent), Enzyme Activity (TBP0020)

Tribioscience Glucose dehydrogenase(NAD(P)-dependent), Enzyme Activity (TBP0020)

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Preparation and Specification

Appearance:White amorphous powder, lyophilized

Activity:Grade 250U/mg-solid or more

Contaminants:NADH oxidase 1.010%, -Glucosidase 1.010% Glucose-6-phosphate dehydrogenase 1.010%

Properties

Stability:Stable at -20C for at least One year

Molecular weight:approx. 101,000 (Gel filtration)

Isoelectric point:4.5

Michaelis constants:NADlinked: 1.3810M (D-Glucose) 3.0910M (NAD)

NADPlinked: 1.2510M (D-Glucose) 4.0710M (NADP)

Inhibitors:Ag, Hg, Monoiodoacetate

Optimum pH:9.0

Optimum temperature:55

pH Stability:pH 6.0-7.5 (20, 16hr)

Thermal stability: 45 (15min-treatment with 50mM K-phosphate buffer, pH 7.0)

Applications

This enzyme is useful for enzymatic determination of D-Glucose